Researchers from Massachusetts General Hospital (including Overall PI, Dr. Patricia Donahoe), Boston Children’s Hospital, Columbia University, the Jackson Laboratory, University of California, San Diego, and the UW Department of Pediatrics, including Co-Investigator David McCulley, MD, were awarded a Program Project Grant from the National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NIH-NICHD) entitled, “Gene Mutation and Rescue in Human Diaphragmatic Hernia.” Dr. McCulley’s role will include creating and analyzing new conditional mouse alleles using CRISPR/Cas9 technology as part of Project 3, which will focus on evaluation of lung and pulmonary vascular phenotypes that result from deletion of genes implicated by genetic and genomic analyses of patients with congenital diaphragmatic hernia (CDH). The UW subaward, in the amount of ~$70,000 in direct costs per year of this 5-year grant, will utilize the UW Genome Editing and Animal Models Core (GEAM) for this work.